研究内容・業績紹介
  
研 究テーマ 
 P型 カチオン輸送ATPアー ゼの作動機構 −小胞体Ca2+-pumpを 中心として− 
  小胞体Ca2+-pumpの 遺伝子異常と病態
  
研究業績
鈴  木  裕
1
Takashi Daiho, Stefania Danko, Kazuo Yamasaki, and Hiroshi Suzuki
Stable Structural Analog of Ca2+-ATPase ADP-insensitive Phosphoenzyme with Occluded Ca2+ Formed by Elongation of A-domain/M1’-linker and Beryllium Fluoride Binding
J.Biol.Chem.  Vol.285, No. 32, 24538-24547, 2010
2
Xiaoyu Liu, Takashi Daiho, Kazuo Yamasaki, Guoli Wang, Stefania Danko, and Hiroshi Suzuki
Roles of Interaction between Actuator- and Nucleotide Binding-domains of Sarco(endo)plasmic Reticulum Ca2+-ATPase as Revealed by Single and Swap Mutational Analyses of Serine186 and Glutamate439
J.Biol.Chem.  Vol.284, No.34, 22722-22735, 2009
3
Stefania Danko, Takashi Daiho, Kazuo Yamasaki, Xiaoyu Liu, and Hiroshi Suzuki
Formation of Stable Structural Analog of ADP-sensitive Phosphoenzyme of Ca2+-ATPase with Occluded Ca2+ by Beryllium Fluoride : Structural Changes during phosphorylation and isomerization
J.Biol.Chem.  Vol.284, No.34, 22722-22735, 2009
4
Kazuo Yamasaki, Guoli Wang, Takashi Daiho, Stefania Danko, and Hiroshi Suzuki
Roles of Tyr122-Hydrophobic Cluster and K+ Binding in Ca2+-releasing Process of ADP-insensitive Phosphoenzyme of Sarcoplasmic Reticulum Ca2+-ATPase
J.Biol.Chem.  Vol.283, No.43, 29144-29155, 2008
5
Takashi Daiho, Kazuo Yamasaki, Stefania Danko, and Hiroshi Suzuki
Critical Role of Glu40-Ser48 Loop Linking Actuator Domain and First Transmembrane Helix of Ca2+-ATPase in Ca2+ Deocclusion and Release from ADP-insensitive Phosphoenzyme
J.Biol.Chem.  Vol.282, No.47, 34429-34447, 2007
6
Yuki Miyauchi, Takashi Daiho, Kazuo Yamasaki, Hidetoshi Takahashi, Akemi Ishida-Yamamoto, Stefania Danko, Hiroshi Suzuki, and Hajime Iizuka
Comprehensive Analysis of Expression and Function of 51 Sarco(endo)plasmic Reticulum Ca2+-ATPase Mutants Associated with Darier Disease
J.Biol.Chem. Vol. 281, No. 32, 22882-22895, 2006
7
Masaki Yoshida, Kazuo Yamasaki, Takashi Daiho, Hajime Iizuka, and Hiroshi Suzuki
ATP2C1 is specifically localized in the basal layer of normal epidermis and its depletion triggers keratinocyte differentiation
J. Dermatol. Science. Vol. 43, No. 1, 21-33, 2006
8
Guoli Wang, Kazuo Yamasaki, Takashi Daiho, and Hiroshi Suzuki
Critical hydorophobic interactions between Phosphorylation-and Actuator-domains of Ca2+-ATPase for hydrolysis of Phosphorylated Intermediate
J.Biol.Chem. Vol. 280, No. 28, 26508-26516, 2005
9
鈴木 裕。  
カルシウムポンプのドメ イン間相互作用変化とエネルギー変換
生物物理 Vol.45(1), 16-21, 2005
10
Katsuhiko Sato, Kazuo Yamasaki, Takashi Daiho, Yuki Miyauchi, Hidetoshi Takahashi, Akemi Ishida-Yamamoto, Satoshi Nakamura, Hajime Iizuka and Hiroshi Suzuki.
Distinct Types of Abnormality in Kinetic Properties of Three Darier Disease-causing Sarco(endo)plasmic Reticulum Ca2+-ATPase (SERCA2b) Mutants, That Exhibit Normal Expression and High Ca2+ Transport Activity.
J.Biol.Chem.Vol.279(34), 35595-35603, 2004 
11
Stefania Danko, Kazuo Yamasaki, Takashi Daiho, and Hiroshi Suzuki.
Distinct Natures of Beryllium Fluoride-bound, Aluminum Fluoride-bound, and Magnesium Fluoride-bound Stable Analogues of an ADP-insensitive Phospoenzyme Intermediate of Sarcoplasmic Reticulum Ca2+-ATPase
J.Biol.Chem. Vol.279(15),14991-14998, 2004
12
Kazuo Yamasaki, Takashi Daiho, Stefania Danko, and Hiroshi Suzuki.
Multiple and  Distinct Effects of  Mutations of  Tyr122,  Glu123, Arg324, and Arg334  Involved in Interactions between the Top Part of Second and Fourh  Transmembrane Helices in Sarcoplasmic Reticulum Ca2+-ATPase.   
J.Biol.Chem.Vol.279(3), 2202-2210, 2004
13
鈴木 裕。  
Ca2+ポンプの構造/ 機能連関および遺伝子異常と細胞病態
生化学vol75(9), 1215-1224, 2003
14
Takashi Daiho, Kazuo Yamasaki, Guoli Wang, Stefania Danko, Hajime Iizuka, and Hiroshi Suzuki.
Delections  of  Any  Single  Residues  in  Glu40 – Ser48  Loop  Connecting  A  Domain  and the  First  Transmembrane  Helix  of  Sarcoplasmic   Reticulum  Ca2+  -ATPase  Result  in  Almost  Complete  Inhibition  of  Conformational  Transition  and  Hydrolysis  of  Phosphoenzyme  Intermediate.
J.Biol.Chem. Vol.278(40), 39197-39204, 2003
15
Sanae Kato, Mika Kamidochi, Takashi Daiho, Kazuo Yamasaki, Guoli Wang, and Hiroshi Suzuki.
Val200  Residue  in  Lys189 – Lys205  Outermost  Loop  on  the  A  Domain  of  Sarcoplasmic  Reticulum  Ca2+ - ATPase  Is  Critical  for  Rapid  Processing  of   Phosphoenzyme  Intermediate  after  Loss  of  ADPSensitivity. 
J.Biol.Chem. Vol278(11), 9624-9629, 2003
16
T.Wada, Y.Shirakata, H.Takahashi, S.Murakami, H.Iizuka, H.Suzuki and K.Hashimoto.
A  Japanese  case  of  segmental  Datier’s  disease  caused  by  mosaicism  for  the  ATP2A2  mutation. 
British Journal of Dermatokogy. Vol.149 , 185-188, 2003
17
Kazuo Yamasaki, Takashi Daiho and Hiroshi Suzuki.
Remarkable Stability of Solubilized and Delipidated Sarcoplasimc Reticulum Ca2+-ATPase with Tightly Bound Fluoride and Magnesium against Detergent-induced Denaturation.
J. Biol. Chem. Vol.227(16),13615-13619 , 2002
18
鈴木 裕。 
筋小胞体Ca2+ポンプのCa2+による分子構造変 化 ――カチオン輸 送エネルギー共役の戦略。
蛋白質核酸酵素 Vol.47(14), 1947-1948, 2002
鈴木 裕。
19
Ca2+ポンプ。
シリーズ・バイオサイエ ンスの新世紀 Vol.12「感覚 器官と脳内情報処理」(日本生化学会・共立出版)、121-1282002
Stefania Danko, Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki and Chikashi Toyoshima.
Organization of cytoplasmic domains of sarcoplasmic reticulum Ca2+-ATPase in E1P and E1ATP states: a limited proteolysis study. 
20
FEBS Letters, Vol.505, 129-135, 2001  (Hiroshi Suzuki; corresponding author)
Daiho, Kazuo Yamasaki, Tomoyuki Saino, Mika Kamidochi, Katsuhiko Satoh, Hajime Iizuka,and Hiroshi Suzuki.
Mutations of Either or both of Cys876 and Cys888 of Sarcoplasmic Reticulum Ca2+-ATPase Resultin a Complete Loss of Ca2+Transport Activity without a Loss of Ca2+-ATPase activity.
.J. Biol. Chem.Vol.276(35), 32771-32778, 2001
21
Hidetoshi Takahashi, Yoshiaki Atsuta, Katsuhiko Sato, Akemi Ishida-Yamamoto, Hiroshi Suzuki, and Hajime Iizuka.
Novel mutations of ATP2A2 gene in Japanese patients of Darier's disease. 
J. Dermatol. Sci. Vol.26(3), 169-172, 2001
22
 鈴木 裕。
骨格筋の収縮・弛緩のス イッチは何か?――興奮収縮連関。
運動と分子生物学Q&A(大修館書店)108-1092001
23
Stefania Danko, Takashi Daiho, Kazuo Yamasaki, Mika Kamidochi, Hiroshi Suzuki, and Chikashi Toyoshima.
ADP-insensitive phosphoenzyme intermediate of sarcoplasmic reticulum Ca2+-ATPase has a compact conformation resistant to proteinase K, V8 protease and trypsin.
FEBS Letters, Vol.498(2-3), 277-282, 2001 (Hiroshi Suzuki; corresponding author)
24
Hiroshi Suzuki, Takashi Daiho, Kazuo Yamasaki, and Tohru Kanazawa.
Only half of the Ca2+-ATPase molecules present in sarcoplasmic reticulum vesicles can be phosphorylated with ATP or Inorganic phosphate.
in Na/K-ATPase and Related ATPases.(K.Taniguchi and S.Kaya Eds.) 
25
pp.381-388, 2000, Elsevier, North Holland,
Takashi Daiho, Kazuo Yamasaki, Hiroshi Suzuki, Tomoyuki Saino, and Tohru Kanazawa.
Deletions or specific substitutions of a few residents in the NH2-terminal Ala3-Thr9 region of sarcoplasmic reticulum Ca2+-ATPase cause inactivation and rapid degradation of the enzyme expressed in COS-1 cells. 
26
in Na/K-ATPase and Related ATPases. (K.Taniguchi and S.Kaya Eds.) 
pp.297-300, 2000, Elsevier, North Holland,
Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki, Tomoyuki Saino, and Tohru Kanazawa.
27
Mutations ofarginine-198 in sarcoplasmic reticulum Ca2+-ATPase cause inhibition of hydrolysis of the phosphoenzyme intermediate formed from inorganic phosphate.
in Na/K-ATPase and Related ATPases. (K.Taniguchi and S.Kaya Eds.) 
pp.297-300, 2000, Elsevier, North Holland,
28
Takashi Daiho, Kazuo Yamasaki, Hiroshi Suzuki, Tomoyuki Saino, and Tohru Kanazawa.
Deletions or Specific Substitutions of a Few Residues in the NH2-terminal Region (Ala3-Thr9) of Sarcoplasmic Reticulum Ca2+-ATPase Cause Inactivation and Rapid Degradation of the Enzyme Expressed in COS-1 Cells. 
J. Biol. Chem. Vol.274(34), 23910-23915, 1999
29
Takashi Daiho, Hiroshi Suzuki, Kazuo Yamasaki, Tomoyuki Saino, and Tohru Kanazawa.
Mutations of Arg198 in Sarcoplasmic Reticulum Ca2+-ATPase Cause Inhibition of Hydrolysis of the Phosphoenzyme Intermediate Formed from Inorganic Phosphate.
FEBS Letters Vol. 444(1), 54-58, 1999
30
Hiroshi Suzuki and Tohru Kanazawa
Formation of the ADP-Insensitive Phosphoenzyme Intermediate in the Sarcoplasmic Reticulum Ca2+-ATPase of Which both Cys344 and Cys364 Are Modified by N-Ethylmaleimide. 
Biochemistry Vol. 38(2), 820-825, 1999
31
鈴木 裕。
Ca2+ポンプ――形質膜と小胞体。
蛋白質核酸酵素Vol.43 (10)(増刊号:カルシウムと シグナル伝達)、1610-16211998
32
Satoshi Nakamura, Hiroshi Suzuki and Tohru Kanazawa
Stoichiometry of Phosphorylation to Fluorescein 5-Isothiocyanate Binding in the Ca2+-ATPase of Sarcoplasmic Reticulum Vesicles.
J. Biol. Chem. Vol.272 (10), 6232-6237, 1997
33
Keisuke Kimura, Hiroshi Suzuki, Takashi Daiho, Kazuo Yamasaki, and Tohru Kanazawa.
Identification of Arginyl Residues Located at the ATP Binding Site of Sarcoplasmic Reticulum Ca2+-ATPase: Modification with 1,2-Cyclohexanedione.
J. Biol. Chem. Vol.271 (46), 28933-28941, 1996
34
Hiroshi Suzuki and Tohru Kanazawa.
Reduction in Water Activity Greatly Retards the Phosphoryl Transfer from ATP to Enzyme Protein in the Catalytic Cycle of Sarcoplasmic Reticulum Ca2+-ATPase.
J. Biol. Chem. Vol.271 (10), 5481-5486, 1996
35
Tohru Kanazawa, Hiroshi Suzuki, Takashi Daiho, and Kazuo Yamasaki.
Fluorometric Study on Conformational Changes in the Catalytic Cycle of Sarcoplasmic Reticulum Ca2+-ATPase. 
Bioscience Reports Vol.15 (5), 317-326, 1995
36
Hiroshi Suzuki and Tohru Kanazawa.
The Tryptophan Fluorescence Change upon Conformational Transition of the Phosphoenzyme Intermediate in Sarcoplasmic Reticulum Ca2+-ATPase Is Revealed in the  Absence of Kand the Presence of Lasalocid. 
J. Biol. Chem. Vol.270 (7), 3089-3093, 1995
37
Hiroshi Suzuki, Satoshi Nakamura, and Tohru Kanazawa.
Effects of Divalent Cations Bound to the Catalytic Site on ATP-Induced Conformational Changes in the Sarcoplasmic Reticulum Ca2+-ATPase: Stopped-Flow Analysis of the  Fluorescence of N-Acetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine Attached to Cysteine-674
Biochemistry Vol.33 (27), 8240-8246, 1994
38
Satoshi Nakamura, Hiroshi Suzuki, and Tohru Kanazawa
The ATP-induced Change of Tryptophan Fluorescence Reflects a Conformational Change upon Formation of ADP-sensitive Phosphoenzyme in the Sarcoplasmic Reticulum Ca2+-ATPase: Stopped-Flow Spectrofluorometry and Continuous Flow-Rapid Quenching Method. 
J. Biol. Chem. Vol.269 (23), 16015-16019, 1994
39
 鈴木 裕。
筋小胞体Ca2+-ATPアーゼ。
生化学64巻(第12号)、1444-14491992
40
Hiroshi Suzuki, Tatsuya Kubota, Koji Kubo, and Tohru Kanazawa
Existence of a Low-affinity ATP-binding Site in the Unphosphorylated Ca2+-ATPase of Sarcoplasmic Reticulum Vesicles: Evidence from Bionding of  2',3'-0-(2,4,6-Trinitrocyclohexandienylidene)-[3H]AMP and -[3H] ATP. 
Biochemistry Vol.29 (30), 7040-7045, 1990
41
Koji Kubo, Hiroshi Suzuki, and Tohru Kanazawa
Characterization of the Substrate-induced Conformational Change of N-Iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine-lebeled Sarcoplasamic Reticulum Ca2+-ATPase by Using Different Kinds of Substrate.
Biochimi. Biophys. Acta Vol.1040(2), 251-259, 1990
42
Suzuki, Mitsuhiro Obara, Koji Kubo, and Tohru Kanazawa.
Changes in the Steady-state Fluorescence Anisotropy of N-Iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine Attached to the Specific Thiol of Sarcoplasmic Reticulum Ca2+-ATPase throughout the Catalytic Cycle.
J. Biol. Chem. Vol.264 (2), 920-927, 1989
43
Hiroshi Suzuki, Yoshifusa Kondo, Aida D. Carlos, and John C. Seidel.
Effects of Phosphorylation, MgATP, and Ionic Strength on the Rates of Papain Degradation of Heavy and Light Chains of Smooth Muscle Heavy Meromyosin at the  S1-S2 Junction.
J. Biol. Chem. Vol.263 (22), 10974-10979, 1988
44
Mithsuhiro Obara, Hiroshi Suzuki, and Tohru Kanazawa.
Conformational Changes in the Vicinity of the N-Iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine Attached to the Specific Thiol of Sarcoplasmic Reticulum Ca2+-ATPase throughout the Catalytic Cycle.
J. Biol. Chem. Vol.263 (8), 3690-3697, 1988 
45
Hiroshi Suzuki, Mitsuhiro Obara, Hideto Kuwayama, and Tohru Kanazawa.
Conformational Changes in the Vicinity of the N-Iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine Attached to the Specific Thiol of Sarcoplasmic Reticulum Ca2+-ATPase through the Catalytic Cycle
Calcium Signal and Cell Response (K. Yagi and T. Miyazaki Eds.) 332-334, 1988
46
Hiroshi Suzuki, Mitsuhiro Obara, Hideto Kuwayama, and Tohru Kanazawa.
A Conformational Change of N-Iodoacetyl-N'-(5-sulfo-1-naphthyul)ethylenediamine-labeled Sarcoplasmic Reticulum Ca2+-ATPase upon ATP Binding to the Catalytic Site.
J. Biol. Chem. Vol.262 (32), 15448-15456, 1987
47
Sumitra Nag, Hiroshi Suzuki, Jan Sosinski, and John C. Seidel.
Conformational Changes in Myosin and Heavy Meromyosin from Chicken Gizzard Associated with Phosphorylation.
Regulation and Contraction of Smooth Muscle (Siegman, M. J., Somlyo, A. P., and Stephens, N. L. Eds.) Vol.91-108, 1987
48
Hiroshi Suzuki, Walter F. Stafford, III, Henry S. Slayter, and John C. Seidel.
A Conformational Transition in Gizzard Heavy Meromyosin Involving the Head-Tail Junction, Resulting in Changes in Sedimentation Coefficient, ATPase Activity, and Orientation of Heads.
J. Biol. Chem. Vol.260, 14810-14817, 1985
49
T. S. Chandra, Narindar Nath, Hiroshi Suzuki, and John C. Seidel.
Modification of Thiols of Gizzard Myosin Alters ATPase Activity, Stability of Myosin Filaments, and the 6-10S Conformational Transition.
J. Biol.Chem. Vol.260, 202-207, 1985
50
鈴木 裕。
平滑筋収縮制御の分子機 構−軽鎖のリン酸化ならびに脱リン酸化によるミオシン分子の形態変化−。
血管「脈管研究」6巻、157-1691983
51
Hiroshi Suzuki, Toshio Kamata, Hirofumi Onishi, and Shizuo Watanabe.
Adenosine Triphosphate-Induced Reversible Change in the Conformation of Chicken Gizzard Myosin and Heavy Meromyosin. 
J. Biochem.Vol.91, 1699-1705, 1982
52
Hiroshi Suzuki, Koui Takahashi, Hirofumi Onishi, and Shizuo Watanabe.
Reversible Changes in the State of Phosphorylation of Gizzard Myosin, in That of Gizzard Myosin Assembly, in the ATPase Activity of Gizzard Myosin, in That of Actomyosin and in the Superprecipitation Activity.
J. Biochem.Vol.91, 1687-1698, 1982
53
Hiroshi Suzuki, Sawako Suzuki, and Shizuo Watanabe.
High Concentrations of Magnesium for Formation of Thick-Filaments of Chicken Gizzard Myosin.
J. Biochem. Vol.89, 871-878, 1981
54
Hiroshi Suzuki, Kunihiko Konno, Ken-ichi Arai, and Shizuo Watanabe
ATP-Induced Tension Development in Glycerinated Fibers of Scallop Adductor Striated Muscle: Role of Regulatory Light Chain of Myosin in Calcium Regulation of Muscle  Contraction.
J. Biochem.Vol.88, 909-911, 1980
55
Hiroshi Suzuki, Hirofumi Onishi, Koui Takahashi, and Shizuo Watanabe
Structure and Function of Chicken Gizzard Myosin. 
J. Biochem. Vol.84, 1529-1542, 1978
56
Hiroshi Suzuki, Kayoko Nakamura, Koui Takahashi, and Shizuo Watanabe.
 Adenosine Triphosphatase Activity and "Thick Filament" Formation of Chicken Gizzard Myosin in Low Salt Media. 
J. Biochem. Vol.83, 835-847, 1978
57
Hirofumi Onishi, Yoosuke Yamada, Mitsuo Ikebe, Hiroshi Suzuki, and Shizuo Watanabe.
The ATPase Reaction in the Steady State and in the Initial Burst Catalyzed by Chicken Gizzard Myosin in 0.6 M KC1. 
J. Biochem. Vol.83, 129-135, 1978
大 保  貴 嗣
1
Takashi Daiho, Stefania Danko, Kazuo Yamasaki, and Hiroshi Suzuki
Stable Structural Analog of Ca2+-ATPase ADP-insensitive Phosphoenzyme with Occluded Ca2+ Formed by Elongation of A-domain/M1’-linker and Beryllium Fluoride Binding
J.Biol.Chem.  Vol.285, No. 32, 24538-24547, 2010
2
Xiaoyu Liu, Takashi Daiho, Kazuo Yamasaki, Guoli Wang, Stefania Danko, and Hiroshi Suzuki
Roles of Interaction between Actuator- and Nucleotide Binding-domains of Sarco(endo)plasmic Reticulum Ca2+-ATPase as Revealed by Single and Swap Mutational Analyses of Serine186 and Glutamate439
J.Biol.Chem.  Vol.284, No.34, 22722-22735, 2009
3
Stefania Danko, Takashi Daiho, Kazuo Yamasaki, Xiaoyu Liu, and Hiroshi Suzuki
Formation of Stable Structural Analog of ADP-sensitive Phosphoenzyme of Ca2+-ATPase with Occluded Ca2+ by Beryllium Fluoride : Structural Changes during phosphorylation and isomerization
J.Biol.Chem.  Vol.284, No.34, 22722-22735, 2009
4
Kazuo Yamasaki, Guoli Wang, Takashi Daiho, Stefania Danko, and Hiroshi Suzuki
Roles of Tyr122-Hydrophobic Cluster and K+ Binding in Ca2+-releasing Process of ADP-insensitive Phosphoenzyme of Sarcoplasmic Reticulum Ca2+-ATPase
J.Biol.Chem.  Vol.283, No.43, 29144-29155, 2008
5
Takashi Daiho, Kazuo Yamasaki, Stefania Danko, and Hiroshi Suzuki
Critical Role of Glu40-Ser48 Loop Linking Actuator Domain and First Transmembrane Helix of Ca2+-ATPase in Ca2+ Deocclusion and Release from ADP-insensitive Phosphoenzyme
J.Biol.Chem.  Vol.282, No.47, 34429-34447, 2007
6
Yuki Miyauchi, Takashi Daiho, Kazuo Yamasaki, Hidetoshi Takahashi, Akemi Ishida-Yamamoto, Stefania Danko, Hiroshi Suzuki, and Hajime Iizuka
Comprehensive Analysis of Expression and Function of 51 Sarco(endo)plasmic Reticulum Ca2+-ATPase Mutants Associated with Darier Disease
J.Biol.Chem. Vol. 281, No. 32, 22882-22895, 2006
7
Masaki Yoshida, Kazuo Yamasaki, Takashi Daiho, Hajime Iizuka, and Hiroshi Suzuki
ATP2C1 is specifically localized in the basal layer of normal epidermis and its depletion triggers keratinocyte differentiation
J. Dermatol. Science. Vol. 43, No. 1, 21-33, 2006
8
Guoli Wang, Kazuo Yamasaki, Takashi Daiho, and Hiroshi Suzuki
Critical hydorophobic interactions between Phosphorylation-and Actuator-domains of Ca2+-ATPase for hydrolysis of Phosphorylated Intermediate
J.Biol.Chem. Vol. 280, No. 28, 26508-26516, 2005
9
Katsuhiko Sato, Kazuo Yamasaki, Takashi Daiho, Yuki Miyauchi, Hidetoshi Takahashi, Akemi Ishida-Yamamoto, Satoshi Nakamura, Hajime Iizuka and Hiroshi Suzuki.
Distinct Types of Abnormality in Kinetic Properties of Three Darier Disease-causing Sarco(endo)plasmic Reticulum Ca2+-ATPase (SERCA2b) Mutants, That Exhibit Normal Expression and High Ca2+ Transport Activity.
J.Biol.Chem. Vol.279(34), 35595-35603, 2004 
10
Stefania Danko, Kazuo Yamasaki, Takashi Daiho, and Hiroshi Suzuki.
Distinct Natures of Beryllium Fluoride-bound, Aluminum Fluoride-bound, and Magnesium Fluoride-bound Stable Analogues of an ADP-insensitive Phospoenzyme Intermediate of Sarcoplasmic Reticulum Ca2+-ATPase
J.Biol.Chem. Vol.279(15),14991-14998, 2004
11
Kazuo Yamasaki, Takashi Daiho, Stefania Danko, and Hiroshi Suzuki.
Multiple and  Distinct Effects of  Mutations of  Tyr122,  Glu123, Arg324, and Arg334  Involved in Interactions between the Top Part of Second and Fourh  Transmembrane Helices in Sarcoplasmic Reticulum Ca2+-ATPase.   
J.Biol.Chem.Vol.279(3), 2202-2210, 2004
12
Takashi Daiho, Kazuo Yamasaki, Guoli Wang, Stefania Danko, Hajime Iizuka, and Hiroshi Suzuki.
Delections  of  Any  Single  Residues  in  Glu40 – Ser48  Loop  Connecting  A  Domain  and the  First  Transmembrane  Helix  of  Sarcoplasmic   Reticulum  Ca2+  -ATPase  Result  in  Almost  Complete  Inhibition  of  Conformational  Transition  and  Hydrolysis  of  Phosphoenzyme  Intermediate. 
J.Biol.Chem. Vol.278(40), 39197-39204, 2003
13
Kato, Mika Kamidochi, Takashi Daiho, Kazuo Yamasaki, Guoli Wang, and Hiroshi Suzuki.
Val200  Residue  in  Lys189 – Lys205  Outermost  Loop  on  the  A  Domain  of  Sarcoplasmic  Reticulum  Ca2+ - ATPase  Is  Critical  for  Rapid  Processing  of   Phosphoenzyme  Intermediate  after  Loss  of  ADPSensitivity. 
J.Biol.Chem. Vol278(11), 9624-9629, 2003
14
Kazuo Yamasaki, Takashi Daiho and Hiroshi Suzuki
Remarkable Stability of Solubilized and Delipidated Sarcoplasimc Reticulum Ca2+-ATPase with Tightly Bound Fluoride and Magnesium against Detergent-induced Denaturation. 
J. Biol. Chem.  Vol.227(16),13615-13619 , 2002
15
Stefania Danko, Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki and Chikashi Toyoshima.
Organization of cytoplasmic domains of sarcoplasmic reticulum Ca2+-ATPase in E1P and E1ATP states: a limited proteolysis study. 
FEBS Letters, Vol.505, 129-135, 2001
16
Takashi Daiho, Kazuo Yamasaki, Tomoyuki Saino, Mika Kamidochi, Katsuhiko Satoh, Hajime Iizuka, and Hiroshi Suzuki.
Mutations of Either or both of Cys876 and Cys888 of Sarcoplasmic Reticulum Ca2+-ATPase Result in a Complete Loss of Ca2+ Transport Activity without a Loss of  Ca2+-ATPase activity, 
J. Biol. Chem. Vol.276(35), 32771-32778, 2001
17
Stefania Danko, Takashi Daiho, Kazuo Yamasaki, Mika Kamidochi, Hiroshi Suzuki, and Chikashi Toyoshima.
ADP-insensitive phosphoenzyme intermediate of sarcoplasmic reticulum Ca2+-ATPase has a compact conformation resistant to proteinase K, V8 protease and trypsin.
FEBS Letters, Vol. 498(2-3), 277-282, 2001
18
Hiroshi Suzuki, Takashi Daiho, Kazuo Yamasaki, and Tohru Kanazawa.
Only half of the Ca2+-ATPase molecules present in sarcoplasmic reticulum vesicles can be phosphorylated with ATP or Inorganic phosphate.
Na/K-ATPase and Related ATPases.(K.Taniguchi and S.Kaya Eds.)Elsevier, North Holland, 381-388, 2000
19
Takashi Daiho, Kazuo Yamasaki, Hiroshi Suzuki, Tomoyuki Saino, and Tohru Kanazawa.
Deletions or specific substitutions of a few residents in the NH2-terminal Ala3-Thr9 region of sarcoprasmic reticulum Ca2+-ATPase cause inactivation and rapid degradation   of the enzyme expressed in COS-1 cells. 
Na/K-ATPase and Related ATPases.(K.Taniguchi and S.Kaya Eds.)Elsevier, North Holland, 293-296, 2000
20
Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki, Tomoyuki Saino, and Tohru Kanazawa.
Mutations of arginine-198 in sarcoplasmic reticulum Ca2+-ATPase cause inhibition of hydrolysis of the phosphoenzyme intermediate formed from inorganic phosphate.
Na/K-ATPase and Related ATPases.(K.Taniguchi and S.Kaya Eds.)Elsevier, North Holland, 297-300, 2000
21
Takashi Daiho, Kazuo Yamasaki, Hiroshi Suzuki, Tomoyuki Saino, and Tohru Kanazawa.
Deletions or Specific Substitutions of a Few Residues in the NH2-terminal Region (Ala3-Thr9) of Sarcoplasmic Reticulum Ca2+-ATPase Cause Inactivation and Rapid Degradation of the Enzyme Expressed in COS-1 Cells. 
J. Biol. Chem. Vol.274 (34), 23910-23915, 1999
22
Takashi Daiho, Hiroshi Suzuki, Kazuo Yamasaki, Tomoyuki Saino, and Tohru Kanazawa.
Mutations of Arg198 in Sarcoplasmic Reticulum Ca2+-ATPase Cause Inhibition of Hydrolysis of the Phosphoenzyme Intermediate Formed from Inorganic Phosphate.
FEBS Letters Vol. 444 (1), 54-58, 1999
23
Kazuo Yamasaki, Takashi Daiho, Tomoyuki Saino, and Tohru Kanazawa.
Modification of Histidine 5 in Sarcoplasmic Reticulum Ca2+-ATPase by Dietyl Pyrocarbonate Causes Strong Inhibition of Formation of Phosphoenzyme Intermediate from  Inorganic Phosphate. 
J. Biol. Chem. Vol.272 (49), 30627-30636, 1997
24
Tomoyuki Saino, Takashi Daiho, and Tohru Kanazawa.
Modification of Arginine-198 in Sarcoplasmic Reticulum Ca2+-ATPase by 1,2-Cyclohexanedione Causes Inhibition of Formation of the Phosphoenzyme Intermediate from  Inorganic Phosphate. 
J. Biol. Chem. Vol.272 (34), 21142-21150, 1997
25
Keisuke Kimura, Hiroshi Suzuki, Takashi Daiho, Kazuo Yamasaki, and Tohru Kanazawa.
Identification of Arginyl Residues Located at the ATP Binding Site of Sarcoplasmic Reticulum Ca2+-ATPase. Modification with 1,2-Cyclohexanedione.
J. Biol. Chem. Vol.271 (46), 28933-28941, 1996
26
Tohru Kanazawa, Hiroshi Suzuki, Takashi Daiho, and Kazuo Yamasaki.
Fluorometric Study on Conformational Changes in the Catalytic Cycle of Sarcoplasmic Reticulum Ca2+-ATPase. 
Bioscience Reports Vol.15 (5), 317-326, 1995
27
Takashi Daiho and Tohru Kanazawa.
Reduction of Disulfide Bonds in Sarcoplasmic Reticulum Ca2+-ATPase by Dithiothreitol Causes Inhibition of Phosphoenzyme Isomerization in Catalytic Cycle : This Reduction  Requires Binding of Both Purine Nucleotide and Ca2+to Enzyme. 
J. Biol. Chem., Vol.269, 11060-11064 , 1994
28
Kazuo Yamasaki, Takashi Daiho, and Tohru Kanazawa
3'-O-(5-Fluoro-2,4-dinitrophenyl)-ATP Exclusively Labels Lys-492 at the Active Site of the Sarcoplasmic Reticulum Ca2+-ATPase.
J. Biol. Chem., Vol.269, 4129-4134, 1994
29
Kazuo Yamasaki, Takashi Daiho, and Tohru Kanazawa.
Labeling of Lysine 492 with Pyridoxal 5'-Phosphate in the Sarcoplasmic Reticulum Ca2+-ATPase : Lysine 492 Residue is Located Outside the Fluorescein  5-Isothiocyanate-Binding Region in or near the ATP Binding Site. 
J. Biol. Chem., Vol.268, 20930-20936, 1993
30
Takashi Daiho, Tatsuya Kubota, and Tohru Kanazawa
Stoichiometry of Tight Binding of Magnesium and Fluoride to Phosphorylation and High-Affinity Binding of ATP, Vanadate, and Calcium in the Sarcoplasmic Reticulum  Ca2+-ATPase. 
Biochemistry, Vol.32, 10021-10026, 1993
31
Tatsuya Kubota, Takashi Daiho, and Tohru Kanazawa
Quasi-irreversible Inactivation of the Sarcoplasmic Reticulum Ca2+-ATPase by Simultaneous Tight Binding of Magnesium and Fluoride to the Catalytic Site.
Biochim. Biophys. Acta, Vol.1163, 131-143, 1993
32
Yamamoto, T., Daiho, T., and Takisawa, H.
Reconstitution of Calcium Transport from Monomeric ATPase of Sarcoplasmic Reticulum.
Perspectives of Biological Energy Transduction,(Mukohata, Y., Morales, M.F., and Fleischer, S.)   147-154, 1987
33
Daiho, T., Takisawa, H., and Yamamoto, T.
Inhibition of hydrolysis of phosphorylated Ca2+,Mg2+-ATPase of the sarcoplasmic reticulum by Ca2+ inside and outside the vesicles.
J. Biochem.(Tokyo) Vol.97(2), 643-653, 1985
山 崎  和 生
1
Takashi Daiho, Stefania Danko, Kazuo Yamasaki, and Hiroshi Suzuki
Stable Structural Analog of Ca2+-ATPase ADP-insensitive Phosphoenzyme with Occluded Ca2+ Formed by Elongation of A-domain/M1’-linker and Beryllium Fluoride Binding
J.Biol.Chem.  Vol.285, No. 32, 24538-24547, 2010
2
Xiaoyu Liu, Takashi Daiho, Kazuo Yamasaki, Guoli Wang, Stefania Danko, and Hiroshi Suzuki
Roles of Interaction between Actuator- and Nucleotide Binding-domains of Sarco(endo)plasmic Reticulum Ca2+-ATPase as Revealed by Single and Swap Mutational Analyses of Serine186 and Glutamate439
J.Biol.Chem.  Vol.284, No.34, 22722-22735, 2009
3
Stefania Danko, Takashi Daiho, Kazuo Yamasaki, Xiaoyu Liu, and Hiroshi Suzuki
Formation of Stable Structural Analog of ADP-sensitive Phosphoenzyme of Ca2+-ATPase with Occluded Ca2+ by Beryllium Fluoride : Structural Changes during phosphorylation and isomerization
J.Biol.Chem.  Vol.284, No.34, 22722-22735, 2009
4
Kazuo Yamasaki, Guoli Wang, Takashi Daiho, Stefania Danko, and Hiroshi Suzuki
Roles of Tyr122-Hydrophobic Cluster and K+ Binding in Ca2+-releasing Process of ADP-insensitive Phosphoenzyme of Sarcoplasmic Reticulum Ca2+-ATPase
J.Biol.Chem.  Vol.283, No.43, 29144-29155, 2008
5
Takashi Daiho, Kazuo Yamasaki, Stefania Danko, and Hiroshi Suzuki
Critical Role of Glu40-Ser48 Loop Linking Actuator Domain and First Transmembrane Helix of Ca2+-ATPase in Ca2+ Deocclusion and Release from ADP-insensitive Phosphoenzyme
J.Biol.Chem.  Vol.282, No.47, 34429-34447, 2007
6
Yuki Miyauchi, Takashi Daiho, Kazuo Yamasaki, Hidetoshi Takahashi, Akemi Ishida-Yamamoto, Stefania Danko, Hiroshi Suzuki, and Hajime Iizuka
Comprehensive Analysis of Expression and Function of 51 Sarco(endo)plasmic Reticulum Ca2+-ATPase Mutants Associated with Darier Disease
J.Biol.Chem. Vol. 281, No. 32, 22882-22895, 2006
7
Masaki Yoshida, Kazuo Yamasaki, Takashi Daiho, Hajime Iizuka, and Hiroshi Suzuki
ATP2C1 is specifically localized in the basal layer of normal epidermis and its depletion triggers keratinocyte differentiation
J. Dermatol. Science. Vol. 43, No. 1, 21-33, 2006
8
Guoli Wang, Kazuo Yamasaki, Takashi Daiho, and Hiroshi Suzuki
Critical hydorophobic interactions between Phosphorylation-and Actuator-domains of Ca2+-ATPase for hydrolysis of Phosphorylated Intermediate
J.Biol.Chem. Vol. 280, No. 28, 26508-26516, 2005
9
Katsuhiko Sato, Kazuo Yamasaki, Takashi Daiho, Yuki Miyauchi, Hidetoshi Takahashi, Akemi Ishida-Yamamoto, Satoshi Nakamura, Hajime Iizuka and Hiroshi Suzuki.
Distinct Types of Abnormality in Kinetic Properties of Three Darier Disease-causing Sarco(endo)plasmic Reticulum Ca2+-ATPase (SERCA2b) Mutants, That Exhibit Normal Expression and High Ca2+ Transport Activity.
J.Biol.Chem. Vol.279(34), 35595-35603, 2004 
10
Stefania Danko, Kazuo Yamasaki, Takashi Daiho, and Hiroshi Suzuki.
Distinct Natures of Beryllium Fluoride-bound, Aluminum Fluoride-bound, and Magnesium Fluoride-bound Stable Analogues of an ADP-insensitive Phospoenzyme Intermediate of Sarcoplasmic Reticulum Ca2+-ATPase
J.Biol.Chem. Vol.279(15),14991-14998, 2004
11
Kazuo Yamasaki, Takashi Daiho, Stefania Danko, and Hiroshi Suzuki.
Multiple and  Distinct Effects of  Mutations of  Tyr122, Glu123, Arg324, and Arg334  Involved in Interactions between the Top Part of Second and Fourh  Transmembrane Helices in Sarcoplasmic Reticulum Ca2+-ATPase.   
J.Biol.Chem.Vol.279(3), 2202-2210, 2004
12
Takashi Daiho, Kazuo Yamasaki, Guoli Wang, Stefania Danko, Hajime Iizuka, and Hiroshi Suzuki.
Delections  of  Any  Single  Residues  in  Glu40 – Ser48  Loop  Connecting  A  Domain  and the  First  Transmembrane  Helix  of  Sarcoplasmic   Reticulum  Ca2+  -ATPase  Result  in  Almost  Complete  Inhibition  of  Conformational  Transition  and  Hydrolysis  of  Phosphoenzyme  Intermediate. 
J.Biol.Chem. Vol.278(40), 39197-39204, 2003
13
Sanae Kato, Mika Kamidochi, Takashi Daiho, Kazuo Yamasaki, Guoli Wang, and Hiroshi Suzuki.
Val200  Residue  in  Lys189 – Lys205  Outermost  Loop  on  the  A  Domain  of  Sarcoplasmic  Reticulum  Ca2+ - ATPase  Is  Critical  for  Rapid  Processing  of   Phosphoenzyme  Intermediate  after  Loss  of  ADPSensitivity. 
J.Biol.Chem. Vol278(11), 9624-9629, 2003
14
Kazuo Yamasaki, Takashi Daiho and Hiroshi Suzuki
Remarkable Stability of Solubilized and Delipidated Sarcoplasimc Reticulum Ca2+-ATPase with Tightly Bound Fluoride and Magnesium against Detergent-induced Denaturation. 
J. Biol. Chem. , Vol.227(16),13615-13619 ,2002 
15
Stefania Danko, Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki and Chikashi Toyoshima.
Organization of cytoplasmic domains of sarcoplasmic reticulum Ca2+-ATPase in E1P and E1ATP states: a limited proteolysis study. 
FEBS Letters, Vol.505, 129-135, 2001
16
Takashi Daiho, Kazuo Yamasaki, Tomoyuki Saino, Mika Kamidochi, Katsuhiko Satoh, Hajime Iizuka, and Hiroshi Suzuki.
Mutations of Either or both of Cys876 and Cys888 of Sarcoplasmic Reticulum Ca2+-ATPase Result in a Complete Loss of Ca2+ Transport Activity without a Loss of Ca2+-ATPase activity, 
J. Biol. Chem. Vol.276(35), 32771-32778, 2001
17
Stefania Danko, Takashi Daiho, Kazuo Yamasaki, Mika Kamidochi, Hiroshi Suzuki, and Chikashi Toyoshima.
ADP-insensitive phosphoenzyme intermediate of sarcoplasmic reticulum Ca2+-ATPase has a compact conformation resistant to proteinase K, V8 protease and trypsin.
FEBS Letters, Vol. 498(2-3), 277-282, 2001
18
Hiroshi Suzuki, Takashi Daiho, Kazuo Yamasaki, and Tohru Kanazawa.
Only half of the Ca2+-ATPase molecules present in sarcoplasmic reticulum vesicles can be phosphorylated with ATP or Inorganic phosphate.
Na/K-ATPase and Related ATPases.(K.Taniguchi and S.Kaya Eds.)Elsevier, North Holland, 381-388, 2000
19
Takashi Daiho, Kazuo Yamasaki, Hiroshi Suzuki, Tomoyuki Saino, and Tohru Kanazawa.
Deletions or specific substitutions of a few residents in the NH2-terminal Ala3-Thr9 region of sarcoprasmic reticulum Ca2+-ATPase cause inactivation and rapid degradation  of the enzyme expressed in COS-1 cells.
Na/K-ATPase and Related ATPases.(K.Taniguchi and S.Kaya Eds.)Elsevier, North Holland, 293-296, 2000
20
Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki, Tomoyuki Saino, and Tohru Kanazawa.
Mutations of arginine-198 in sarcoplasmic reticulum Ca2+ATPase cause inhibition of hydrolysis of the phosphoenzyme intermediate formed from inorganic phosphate.
Na/K-ATPase and Related ATPases.(K.Taniguchi and S.Kaya Eds.)Elsevier, North Holland, 297-300, 2000
21
Takashi Daiho, Kazuo Yamasaki, Hiroshi Suzuki, Tomoyuki Saino, and Tohru Kanazawa.
Deletions or Specific Substitutions of a Few Residues in the NH2-terminal Region (Ala3-Thr9) of Sarcoplasmic Reticulum Ca2+-ATPase Cause Inactivation and Rapid  Degradation of the Enzyme Expressed in COS-1 Cells. 
J. Biol. Chem. Vol.274 (34), 23910-23915, 1999
22
Takashi Daiho, Hiroshi Suzuki, Kazuo Yamasaki, Tomoyuki Saino, and Tohru Kanazawa.
Mutations of Arg198 in Sarcoplasmic Reticulum Ca2+-ATPase Cause Inhibition of Hydrolysis of the Phosphoenzyme Intermediate Formed from Inorganic Phosphate.
FEBS Letters Vol. 444 (1), 54-58, 1999
23
Kazuo Yamasaki, Takashi Daiho, Tomoyuki Saino, and Tohru Kanazawa.
Modification of Histidine 5 in Sarcoplasmic Reticulum Ca2+-ATPase by Dietyl Pyrocarbonate Causes Strong Inhibition of Formation of Phosphoenzyme Intermediate from  Inorganic Phosphate
J. Biol. Chem. Vol.272 (49), 30627-30636, 1997
24
Keisuke Kimura, Hiroshi Suzuki, Takashi Daiho, Kazuo Yamasaki, and Tohru Kanazawa.
Identification of Arginyl Residues Located at the ATP Binding Site of Sarcoplasmic Reticulum Ca2+-ATPase. Modification with 1,2-Cyclohexanedione.
J. Biol. Chem. Vol.271 (46), 28933-28941, 1996